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ATCC® Number:PCS-100-013
Name: Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Umbilical vein endothelium
Growth Properties: Adherent
Age: Neonatal
Morphology: Cobblestone appearance
Number of Viable Cells per Vial: ≥ 500,000 in cryopreservation medium
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Vascular Cell Basal Medium (ATCCPCS-100-030)Endothelial Cell Growth Kit/BBE (ATCCPCS-100-040)Endothelial Cell Growth Kit/VEGF (ATCCPCS-100-041)Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)Trypsin Neutralizing Solution (ATCCPCS-999-004)Gentamicin-Amphotericin B Solution (ATCCPCS-000-025)Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)Phenol Red (ATCCPCS-999-001)
Comments:The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. ATCCprimary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors. Large lot sizes assure consistency for large-scale studies. A complete solution to propagate pooled HUVECs in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more authentic, less defined culture medium is desired.
Applications:Drug screening, angiogenesis, tubule formation, immune response, arteriolosclerosis, hypertension, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, oxidative stress, apoptosis, stent development
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests:Bacteria and Yeast: Negative
Mycoplasma: Negative