兔抗ZYX多克隆抗体

Background:

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Applications:

ELISA, WB, IHC

Name of antibody:

ZYX

Immunogen:

Fusion protein of human ZYX

Full name:

zyxin

Synonyms:

ESP-2; HED-2

SwissProt:

Q15942

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human colorectal cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

61 kDa

WB Positive control:

293T cell lysate

WB Recommended dilution:

200-1000